Specific detection of Mycobacterium tuberculosis by mtp40 nested PCR
نویسندگان
چکیده
منابع مشابه
Detection of Ethambutol-Resistant Associated Mutations in Mycobacterium tuberculosis Isolates from Iran Using Multiplex Allele-Specific PCR
Tuberculosis is a serious global public health problem and its high prevalence is stron gly associated with the enhancement of drug resistance. In this study we demonstrate a multiplex allele-specific polymerase chain reaction (MAS)-PCR assay to simultaneously detect mutations in the first and third bases of the embB gene codon 306 ATG in ethambutol (EMB) resistant isolates of Mycobacterium t...
متن کاملPerformance of nested PCR in the specific detection of Mycobacterium tuberculosis complex in blood samples of pediatric patients.
OBJECTIVE To evaluate the performance of nested PCR (nPCR) in detecting the Mycobacterium tuberculosis complex in blood samples of patients suspected of having TB, in order to determine its potential for use as an auxiliary tool in the laboratory diagnosis of TB in children. METHODS Detection of the M. tuberculosis complex in blood samples using as a target the insertion sequence IS6110 of th...
متن کاملDifferentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments
BACKGROUND Control of the global burden of tuberculosis is obstructed due to lack of simple, rapid and cost effective diagnostic techniques that can be used in resource poor-settings. To facilitate the early diagnosis of TB directly from clinical specimens, we have standardized and validated the use of nested multiplex PCR, targeting gene fragments IS6110, MTP40 and 32kD α-antigen encoding gene...
متن کاملDetection of Mycobacterium tuberculosis from sputum specimens using one-tube nested PCR.
One-tube nested PCR was developed for diagnosis of pulmonary tuberculosis using sequences based on thel6SrRNA gene. The usage of primers 16SOL, 16SOR, 16SIL and 16SIR with optimized conditions could detect 555 bp DNA band from 21 species, 41 strains of mycobacteria and one isolate of Nocardia asteroides. It also revealed a specific 306 bp DNA band from 59 strains of M. tuberculosis complex. Cro...
متن کاملDirect detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventi...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.11.2866-2867.1996